Figure 4

The free energy values in kcal/mol for e and i donor of human (a) confirmed GTNGTNs and (b) unconfirmed GTNGTNs. In (a) black triangles represent tandem donors with more ESTs for the e donor, and blue crosses tandem donors with more ESTs for the i donor. In (b) black triangles represent annotated e donors, and blue crosses annotated i donors. To better illustrate the distribution of the free energies, we added a random number between -0.1 and 0.1 to each value (necessary since many donor sites have the same 9 nucleotide context pattern).