Relative contribution of POFs to biological functions. (a) Representation of POFs in EST libraries from different organisms (A. thaliana, At; O. sativa, Os; D. melanogaster, Dm; A. gambiae, Ag; M. musculus, Mm; R. norvegicus, Rn; C. elegans, Ce; H. sapiens, Hs). (b) Representation of POFs in protein-protein interaction networks in Sc and Ce. (c) Percent phenotypic penetrance of PDFs and POFs in Sc. EST libraries, protein-protein interaction data, insertional mutagenesis data, and sequence comparisons were obtained/performed as described in Materials and methods.