Figure 5
From: Slow, stochastic transgene repression with properties of a timer
Repression of YFP by clones 6 and 18 with varied culture passaging conditions. (a) Levels of YFP fluorescence, high, low, and none, were defined by flow cytometry profiles of untransduced cells (No GFP, No YFP), cells transduced with GFP only, cells transduced with YFP only, and clones 6 and 18 transduced with YFP. (b) Fraction of cells with high YFP fluorescence. X-axis, time in days. When passaging cultures daily, different fractions of culture were retained: one-half (diamonds), one-third (squares), one-quarter (triangles), one-fifth (circles). (c) Rates of YFP repression for clones 6 (gray) and 18 (white).