Figure 2

GFP fluorescence of clones 5, 6, and 18. (a) Levels of GFP fluorescence, high, low, and none, were defined by flow cytometry profiles of untransduced cells (No GFP) and clones 5, 6, and 18. X-axis, relative fluorescence; Y-axis, number of cells. (b) GFP fluorescence: top, during initial expansion from a single cell and subsequent passaging (no error bars because there was only one replicate, that is, n = 1); middle, fluorescence in cells isolated from the first experiment (top) (clone 5 without error bars because n = 1); bottom, fluorescence in cells isolated from the second experiment (middle). Fraction of cells are shown with high (diamonds), low (squares), or no (triangles) fluorescence.