Figure 1
From: Slow, stochastic transgene repression with properties of a timer
Expression of GFP fluorescence in clones. (a) Clones were transduced with a retroviral vector. LTR, long terminal repeat; ψ, retroviral packaging signal; GFP, green fluorescent protein; IRES, internal ribosome entry site; PuroR, puromycin resistance gene. (b) Flow cytometry profiling GFP fluorescence of untransduced cells (No GFP) and clones 1 to 24, which were transduced with the GFP vector (other clones in Additional data file 4). X-axis, relative fluorescence, where that of untransduced cells was set to 3.0; Y-axis (from front to back), 13, 22, 32, and 42 days in culture; Z-axis, normalized cell number; upper left corners show the clone identification number followed by the fraction of the population with fluorescence less than 100 after 32 days. (c) Histogram summary of GFP repression in clones after 32 days. Bars represent number of clones transduced with GFP (gray) and clones transduced with GFP plus an adjacent Ig enhancer (black).