Figure 7From: RNA interference is not involved in natural antisense mediated regulation of gene expression in mammalsIFNβ and OAS2 mRNA expression. The interferon response was quantified by qRT-PCR in: HeLa cells transfected with IVT-dsRNA of HIF (1) or IVT-dsRNA of TS (2); HeLa cells overexpressing S-AS of TS (3); or reference HeLa cells (4). The mRNA levels were normalized to glyceraldehydes 3-phosphate dehydrogenase. In cells transfected with duplex RNA originated from TS or HIF, there was a remarkable increase in IFN-β and OAS2 mRNA.Back to article page