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Figure 1 | Genome Biology

Figure 1

From: RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals

Figure 1

Thymidylate synthase genomic location. (a) Schematic presentation of TS sense and rTSα antisense mRNA. Exons are presented as boxes and the location of probes used for in situ hybridization (ISH) as well as the 3' overlap region of both sense and antisense mRNA are also indicated. The entire overlap region of both sense and antisense mRNA (red hash shaded region) were cloned into the vector described in (b). (b) Conformation of vectors used for transfection and S-AS RNA production. The sense vector makes luciferase RNA with a 3' sense overlap sequence, the antisense vector makes an analogous RNA with a 3' antisense overlap region, and the S-AS vector makes RNA with a consecutive sense-antisense sequence with a hairpin sequence between them.

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