Neighbor-joining tree showing the family-specific amplification of NBS sequences. (a) TNLs. (b) CNLs. The complete tree was based on 1,600 sequences (see Additional data files 1 and 2 for an expanded tree with individual sequences and the alignments used). Clades that contained sequences from individual plant families were collapsed into single branches and the number of sequences in each branch is indicated. Different taxa are assigned different colors; clades with representatives from several families are shown in black. The scale bar represents five nucleotide substitutions.