Skip to main content
Figure 2 | Genome Biology

Figure 2

From: The contributions of normal variation and genetic background to mammalian gene expression

Figure 2

Reproducibility of variant and invariant gene expression characteristics. Quantitative RT-PCR measurements of Cth, CisH, and S16 transcript levels are shown for the original three mice used in the microarray experiments (blue squares), and a second cohort of three additional mice from each strain (gray triangles). The original mouse measurements for Cth and S16 are the same as in Figure 1c, but presented here in log2 scale. Mice in the second cohort underwent 4 independent RNA preparations from each liver (total of 12 RNA preparations per strain). Error bars represent the standard deviation of transcript measurements from the four RNA preparations, or from four replicate PCR reactions in the case of the original mice (note that for some measurements, the error bars fall within the square). Quantitative RT-PCR measurements of Apoa4, Dnase2a, and Socs2 from the additional mice are shown in Additional data file 2 (supplemental Figure 1).

Back to article page