Figure 6
From: Ranked prediction of p53 targets using hidden variable dynamic modeling
Small interfering (si)RNAp53 reduces p53 protein levels and transcription of p53 target genes. (a) Transfection of siRNAp53 reduces p53 protein levels below control values. (b) Real-time quantitative polymerase chain reaction measurement of three p53 target genes (GADD45α, p21, and HDM2) and a control gene (GAPDH) after transfection of siRNAp53 and irradiation. IR, ionizing irradiation.