Table 2 Number of raw sequences, selected ESTs, sizes, contigs formed, and redundancy in A. pisum EST database
From: Large-scale gene discovery in the pea aphid Acyrthosiphon pisum(Hemiptera)
Biological source | Library | EST | Rejected | Selected | M bp | Contig | Singletons | Redundancy | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
Bacterial | rRNA | Short sequences | Vector sequences | ||||||||
Antennae | ApAL3SD | 1,031 | 10 | 39 | 84 | 0 | 898 | 398 | 305 | 283 | 34.52 |
ID0AEE | 5,424 | 23 | 431 | 46 | 1 | 4,923 | 622 | 1,037 | 2,414 | 29.90 | |
Bacteriocyte | ApBac | 2,345 | 1 | 0 | 3 | 0 | 2,341 | 871 | 275 | 40 | 86.54 |
Digestive tract | ApDT | 1,184 | 52 | 333 | 94 | 0 | 705 | 403 | 267 | 211 | 32.20 |
ApHL3LD | 1,245 | 24 | 30 | 359 | 0 | 832 | 394 | 366 | 201 | 31.85 | |
Head | ApHL3SD | 2,068 | 7 | 33 | 739 | 0 | 1,289 | 363 | 382 | 438 | 36.38 |
ID0ACC | 10,706 | 3 | 902 | 221 | 3 | 9,577 | 574 | 2,012 | 1,564 | 62.66 | |
Parthenogenetic embryo | ID0ADD | 5,473 | 136 | 541 | 105 | 0 | 4,691 | 717 | 210 | 151 | 92.30 |
Whole body, multistage | ApMS; 14419; 14436 | 17,964 | 479 | 1455 | 382 | 0 | 15,648 | 716 | 5153 | 3027 | 47.72 |
GenBank | mRNA | 3 | 0 | 0 | 0 | 0 | 3 | 1220 | 2 | 1 | 0.00 |
Total | 47,443 | 735 | 3,764 | 2,033 | 4 | 40,907 | 628 | 4,300 | 7,782 | 70.46 | |