Lineage-specific expansion of proteins exported to erythrocytes in malaria parasites

Table 2 P. falciparum gene families encoding exported proteins

Family	Paralogs	Transmembrane domains	Exons	Microarray data						Comments
				R	T	S	M	Sp	G
PfEMP1	59	1	2	x	x	x	x	x	x
DnaJ I	3	0	1/4/5	1	0	0	1	0	0	DnaJ domains 1-3
DnaJ III	16	0/1	2	3	1	1	3	0	0	DnaJ domain 1
EMP3	2	0	2	2	2	1	2	1	0	MC proteins
GBP130	3	0	2	2	2	2	1	1	0	RBC surface proteins
FIKK kinases	20	0	3	4	4	0	4	0	0	Protein kinase domain
PfMC-2TM	10	2	2	0	2	0	0	0	0	MC proteins
RIFIN	160	2	2	3	6	2	2	1	0	RBC surface proteins
STEVOR	30	2	2	0	0	0	0	0	0	MC proteins
a/b_HYDa	4	1 × 2, 2 × 1	1 × 1, 1 × 7, 2 × 2	0	0	0	0	0	0	a/b hydrolases
a/b_HYDb	4	0	2 × 1, 2 × 2	0	1	1	0	0	0	a/b hydrolases
HYP1	2	0	2	1	1	0	1	0	0
HYP2	3	1 × 2, 2 × 0	1 × 1, 2 × 2	0	0	0	0	0	0	Probably not a real family
PHISTa	23	0	2/3	2	1	2	2	1	1	Four alpha helices
PHISTb	23	0	2	13	10	6	6	2	3	Four alpha helices
PHISTc	16	0	2	9	4	2	7	1	0	Four alpha helices conserved
HYP4	8	1	2	0	0	0	0	0	0	Similar to HYP6
HYP5	7	1	2	0	0	0	0	0	0
HYP6	3	2	2	0	0	0	0	0	0
HYP7	3	2	2	0	0	0	0	0	0	Similar to HYP8
HYP8	2	2	2	2	1	0	2	0	0	Proteomics iRBC localisation
HYP9	5	1	2	1	2	0	0	0	0
HYP10	2	1	2	0	0	0	0	0	0	May be GPI anchored
HYP11	5	0	2 × 1, 3 × 2	0	1	0	0	0	0	Similar to PHIST
HYP12	3	0	2	2	1	1	1	0	0
HYP13	2	2	2	1	0	0	1	0	0	Similar to HYP5
HYP15	4	2	2 × 1, 2 × 2	0	0	0	1	0	0	Similar to HYP5
HYP16	2	1 × 1, 1 × 2	2	1	0	0	1	0	0	Conserved
HYP17	2	1	2	1	0	0	0	0	0

Approximately 75% of all 396 P. falciparum proteins predicted to be exported are organised in families. Counts in columns 5 to 10 represent the number of family members deemed to be expressed by this method for each life cycle stage (except PfEMP1). Abbreviations for life cycle stages in the microarray section are: R, ring; T, trophozoite; S, schizont; M, merozoite; Sp, sporozoite; G, gametocyte. GPI, glycosylphosphatidyl inositol; iRBC, infected red blood cell; MC, Maurer's clefts; RBC, red blood cell.

ISSN: 1474-760X