DNAJ phylogeny and gene/domain models. (a) The inset shows the complete tree of DnaJ domain sequences extracted from different Plasmodium species and C. hominis. Subtrees containing domains from exported (b) type I and (c) type III proteins are highlighted. DnaJ subtrees (type I, bootstrapped neighbour joining on full sequences; type III, TREE-PUZZLE on full sequences; branches with <50% bootstrap support in red, 50% to 75% in blue, >75% in black) show export (blue boxes) and core motif variations. Sequences not belonging to P. falciparum are marked with symbols to indicate the species group from which they arise. PF14_0359 has orthologs in other Plasmodia (not shown). Positions of signal sequence and PEXEL as determined by ExportPred, and PHIST and DnaJ domains as determined by HMM search, are overlaid on gene models. Although the large number of family members tends to decrease bootstrap support for individual branches, congruence with the three PHIST subfamilies as defined by HMM profiles lends additional support to the overall topology of the tree.