Alternate transcription of the Toll-like receptor signaling cascade

Table 1 Summary of splicing microarray data: unstimulated macrophages versus macrophages subjected to seven hours of LPS stimulation

	Expressed unstimulated macrophages	Expressed macrophages + 7 hr LPS	Variants repressed by LPS/Tlr4 (t test P < 0.05)	Variants induced by LPS/Tlr4 (t test, P < 0.05)
Transcript framework	107	106	13	26
Total number of probes	1,404	1,376	39	88
Receptors	16 TK		Csf1r, Ifnar1	Tlr3, Cd86, Clesf9
Adapters	13 TK		Tollip	Rac2
Signal transducers	30 TK		Irak4, Map3k1, Map3k7, Mapk14, Pik3cg, Serpinb3b	Pde4a, Pik3cd, Tbk1
Transcription factors	29 TK		Atf2, Dnmt1, Sumo	Nfkbia, Nfkb1, Nfkb2, Ikbke, Irf1, Stat1, Stat2, Stat3, Sin3a
Inflammatory effectors	17 TK		Serpinb3b	Ccl3, Ccl4, Ccl5, Cxcl10, Cxcl9, Il12b, Il1b, Il6, Casp8, Tnf-α

The splicing array consisted of 1,717 oligonucleotide probes representing alternate transcripts arising from 108 TK. The experiment was repeated four times, with dye-swap. LPS, lipopolysaccharide; TK, transcriptional framework; Tlr, Toll-like receptor.

ISSN: 1474-760X