Mapk14/p38-α isoforms differentially alter macrophage survival. Raw264.7 cells were transfected with (a) pef6 vector only, (b) mitogen-activated protein kinase (Mapk)14 variant 1, and (c) Mapk14 variant 3. Each panel illustrates the transfectants before (left panels) and after (right panels) 6 hours of exposure to lipopolysaccharide (LPS; 10 ng/ml). Fluorescence activated cell sorting (FACS) analysis showed the percentage of cells in each stage of the cell cycle: apoptosis (A), G1, S, and G2 phases. The FACS findings in panel a were as follows: unstimulated pef6 control - A 2.4, G1 69, S 17.6, and G2 10.9; and +LPS - A 3.1, G1 78.6, S 9.2, and G2 9.4. In (b) the FACS findings show that Mapk14 isoform 1 lowered inflammatory cell survival: FACS before - A 3.5, G1 74, S 12, and G2 10.6; and +LPS - A 20.5, G1 59.5, S 12, and G2 8.2. In (c) the FACS findings show that Mapk14 isoform 3 over-expression alters cell survival in unstimulated and inflammatory cells: FACS before - A 9, G1 65, S 8, and G2 18; and +LPS - A 32.5, G1 47.5, S 7, and G2 13.