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Table 3 Detailed overview of the induced mutations retrieved from the mutant medaka library

From: Generation of medaka gene knockout models by target-selected mutagenesis

Dicer (Dcr-1)
Number Exon Sequence context Amino acid change Type of mutation
1 10_11 5'-GATCCTTAGG (A>G) ACAAATGCTC-3' N578D Substitution
2 10_11 5'-GTGGTTGACG (A>G) TGACAACATC-3' D597G Substitution
3 10_11 5'-ACCGTCAACA (C>A) AGCCATCGGT-3' T619K Substitution
4 10_11 5'-CGTCAACACA (G>A) CCATCGGTCA-3' A620T Silent
5 10_11 5'-CAGGTACCTG (C>T) CCTGCTTTGA-3'   Intron
6 10_11 5'-CCTGCCCTGC (T>A) TTGATGTGGA-3'   Intron
7 10_11 5'-AGAATTAACT (T>A) CAACTCAACA-3'   Intron
8 16_17 5'-ATTTTTGACT (T>A) GAATAGTTGG-3'   Intron
9 16_17 5'-GAGGCTCGCA (C>T) TGGCATTCCT-3' T897I Substitution
10 16_17 5'-CGCACTGGCA (T>G) TCCTACCACT-3' I899S Substitution
11 16_17 5'-ACTACCAGGA (C>A) GCTGTCATCA-3' D919E Substitution
12 16_17 5'-GCTCCTTCAG (T>A) GAAACTCTTG-3'   Intron
13 16_17 5'-TCTCCATAGA (T>A) ATCGTAACTT-3' Y926N Substitution
14 16_17 5'-CCATAGATAT (C>T) GTAACTTTGA-3' R927C Substitution
15 16_17 5'-GCCACTCAGC (A>G) AGTTTCCTTC-3' K949E Substitution
16 16_17 5'-TTCCTTCACC (A>T) GAATACGAGA-3' P953P Silent
17 16_17 5'-ACCTGTCAAA (T>A) CTGAACCAGC-3' N972K Substitution
18 20a 5'-GGTTTTTGTG (T>C) CAGATATCCA-3'   Intron
19 20a 5'-CCATTGACAA (C>A) AAAGCTTACA-3' N1094K Substitution
20 20a 5'-AAGCTTACAG (T>A) TCTTGCTCCG-3' S1098R Substitution
21 20a 5'-TTGCTCCGAG (T>C) CCTGCAGCGA-3' S1103P Substitution
22 20a 5'-GCTCAGAACC (T>G) GCCCTCTCAG-3' P1120P Silent
23 20a 5'-CCTTCACCAA (C>T) CTGACAGCTG-3' P1168S Substitution
24 22b 5'-AATAAGGCCT (A>G) CCTGCTGCAA-3' Y1635C Substitution
25 25_26 5'-AGGAAGAGGA (C>T) ATTGAGGTCC-3' D1754D Silent
26 25_26 5'-TTCATCACTG (T>A) TGTTGGAGAT-3'   Intron
27 25_26 5'-CTGCTGGAGA (T>A) GGAGCCGGAA-3' M1813K Substitution
p53
1 5_6_7 5'-TCCCTTTTCT (C>T) CATCGACTGT-3'   Intron
2 5_6_7 5'-TGGCCCAGTA (T>A) TTTGAAGACC-3' Y186X Truncation
3 5_6_7 5'-CTACATGTGT (A>G) ACAGCTCGTG-3' N220D Substitution
4 5_6_7 5'-TACATGTGTA (A>G) CAGCTCGTGC-3' N220S Substitution
5 5_6_7 5'-GTGTAACAGC (T>C) CGTGCATGGG-3' S222P Substitution
6 5_6_7 5'-TCTGGAAACC (G>T) AGTAAGTTTA-3' E241X Truncation
7 5_6_7 5'-GGAAACCGAG(T>C)AAGTTTAGTC-3'   Splice
Sirt1
1 2_3_4 5'-CGATGACGGA (T>A) CCTCTCATGC-3' S138T Substitution
2 2_3_4 5'-CTAGTTCCAG (C>G) GACTGGACTC-3' S144R Substitution
3 2_3_4 5'-AGTTCCAGCG (A>G) CTGGACTCCG-3' D145G Substitution
4 2_3_4 5'-AGCGACTGGA (C>T) TCCGCAGCCC-3' T147I Substitution
5 2_3_4 5'-CAGCCCCAGA (T>A) CGGTCAGAAT-3' I152N Substitution
6 2_3_4 5'-AAGCCGTTGT (G>T) AGCTCAGGTG-3'   Intron
7 2_3_4 5'-CCCGAGACCA (T>C) ACTCCCACCC-3' I179T Substitution
8 2_3_4 5'-CTGTGGCAGA (T>C) CATCATCAAC-3' I192T Substitution
9 2_3_4 5'-ATCATGGTTC (T>C) GACCGGTGCA-3' L227P Substitution
10 2_3_4 5'-CGGTGCAGGT (G>T) TAGGTGTTTC-3'   Splice
11 2_3_4 5'-TAAAGAAACG (G>A) TAAACACCGG-3'   Intron
12 2_3_4 5'-CGGCTTGCTG (T>C) CGACTTTCCC-3' V253A Substitution
13 5_6 5'-AACATCGACA (C>A) GCTGGAACAA-3' T317K Substitution
14 5_6 5'-TGCGACGGCT (T>C) CCTGTCTCGT-3' S338P Substitution
15 5_6 5'-CGTTTGTAAA (C>A) ACAAAGTGGA-3' H344N Substitution
Blm
1 5_6 5'-AGCAGTAGGG (C>T) AATCTGTGTG-3' A477V Substitution
2 5_6 5'-TGTGACTCTC (T>G) ATCAACTCCC-3' L489R Substitution
3 5_6 5'-ACTTCTAAAA (C>T) AACCTTGTTT-3' Q497X Truncation
4 5_6 5'-TTTCTCAGAG (A>G) GCACAAGTCG-3' S503G Substitution
5 7 5'-TATTTTCTAT (C>T) TTCATTCAGA-3'   Intron
6 7 5'-CTTGATGCCC (A>G) CAGGTTGGTG-3' T670A Substitution
Parkin ( Park2 )
1 9_10_11 5'-ATGCACGGTA (C>G) CAGCAATATG-3' Y314X Truncation
2 9_10_11 5'-GACTCATGTG (T>C) CCGGCACCTG-3' C331C Silent
3 9_10_11 5'-AGGGTGGAGT (G>T) TGAGAGACAG-3' C351F Substitution
4 9_10_11 5'-GCTGTGGCTT (T>A) GTCTTCTGTA-3' F359L Substitution
5 9_10_11 5'-TTTTGTGATG (A>T) CATTGCCGTG-3'   Intron
6 9_10_11 5'-GTCTTATTCA (G>A) GAGATGACCA-3' Q410Q Silent
7 9_10_11 5'-TCTCCACCTG (C>T) AGGTGGCTGC-3'   Intron
8 9_10_11 5'-TGCACATGCA (T>C) TGTGCTCTGT-3' H433H Silent
9 9_10_11 5'-AGGGAGTGCA (T>A) GGGAAACCAC-3' M454K Substitution