Table 3 Detailed overview of the induced mutations retrieved from the mutant medaka library
From: Generation of medaka gene knockout models by target-selected mutagenesis
Dicer (Dcr-1) | ||||
|---|---|---|---|---|
Number | Exon | Sequence context | Amino acid change | Type of mutation |
1 | 10_11 | 5'-GATCCTTAGG (A>G) ACAAATGCTC-3' | N578D | Substitution |
2 | 10_11 | 5'-GTGGTTGACG (A>G) TGACAACATC-3' | D597G | Substitution |
3 | 10_11 | 5'-ACCGTCAACA (C>A) AGCCATCGGT-3' | T619K | Substitution |
4 | 10_11 | 5'-CGTCAACACA (G>A) CCATCGGTCA-3' | A620T | Silent |
5 | 10_11 | 5'-CAGGTACCTG (C>T) CCTGCTTTGA-3' | Â | Intron |
6 | 10_11 | 5'-CCTGCCCTGC (T>A) TTGATGTGGA-3' | Â | Intron |
7 | 10_11 | 5'-AGAATTAACT (T>A) CAACTCAACA-3' | Â | Intron |
8 | 16_17 | 5'-ATTTTTGACT (T>A) GAATAGTTGG-3' | Â | Intron |
9 | 16_17 | 5'-GAGGCTCGCA (C>T) TGGCATTCCT-3' | T897I | Substitution |
10 | 16_17 | 5'-CGCACTGGCA (T>G) TCCTACCACT-3' | I899S | Substitution |
11 | 16_17 | 5'-ACTACCAGGA (C>A) GCTGTCATCA-3' | D919E | Substitution |
12 | 16_17 | 5'-GCTCCTTCAG (T>A) GAAACTCTTG-3' | Â | Intron |
13 | 16_17 | 5'-TCTCCATAGA (T>A) ATCGTAACTT-3' | Y926N | Substitution |
14 | 16_17 | 5'-CCATAGATAT (C>T) GTAACTTTGA-3' | R927C | Substitution |
15 | 16_17 | 5'-GCCACTCAGC (A>G) AGTTTCCTTC-3' | K949E | Substitution |
16 | 16_17 | 5'-TTCCTTCACC (A>T) GAATACGAGA-3' | P953P | Silent |
17 | 16_17 | 5'-ACCTGTCAAA (T>A) CTGAACCAGC-3' | N972K | Substitution |
18 | 20a | 5'-GGTTTTTGTG (T>C) CAGATATCCA-3' | Â | Intron |
19 | 20a | 5'-CCATTGACAA (C>A) AAAGCTTACA-3' | N1094K | Substitution |
20 | 20a | 5'-AAGCTTACAG (T>A) TCTTGCTCCG-3' | S1098R | Substitution |
21 | 20a | 5'-TTGCTCCGAG (T>C) CCTGCAGCGA-3' | S1103P | Substitution |
22 | 20a | 5'-GCTCAGAACC (T>G) GCCCTCTCAG-3' | P1120P | Silent |
23 | 20a | 5'-CCTTCACCAA (C>T) CTGACAGCTG-3' | P1168S | Substitution |
24 | 22b | 5'-AATAAGGCCT (A>G) CCTGCTGCAA-3' | Y1635C | Substitution |
25 | 25_26 | 5'-AGGAAGAGGA (C>T) ATTGAGGTCC-3' | D1754D | Silent |
26 | 25_26 | 5'-TTCATCACTG (T>A) TGTTGGAGAT-3' | Â | Intron |
27 | 25_26 | 5'-CTGCTGGAGA (T>A) GGAGCCGGAA-3' | M1813K | Substitution |
p53 | ||||
1 | 5_6_7 | 5'-TCCCTTTTCT (C>T) CATCGACTGT-3' | Â | Intron |
2 | 5_6_7 | 5'-TGGCCCAGTA (T>A) TTTGAAGACC-3' | Y186X | Truncation |
3 | 5_6_7 | 5'-CTACATGTGT (A>G) ACAGCTCGTG-3' | N220D | Substitution |
4 | 5_6_7 | 5'-TACATGTGTA (A>G) CAGCTCGTGC-3' | N220S | Substitution |
5 | 5_6_7 | 5'-GTGTAACAGC (T>C) CGTGCATGGG-3' | S222P | Substitution |
6 | 5_6_7 | 5'-TCTGGAAACC (G>T) AGTAAGTTTA-3' | E241X | Truncation |
7 | 5_6_7 | 5'-GGAAACCGAG(T>C)AAGTTTAGTC-3' | Â | Splice |
Sirt1 | ||||
1 | 2_3_4 | 5'-CGATGACGGA (T>A) CCTCTCATGC-3' | S138T | Substitution |
2 | 2_3_4 | 5'-CTAGTTCCAG (C>G) GACTGGACTC-3' | S144R | Substitution |
3 | 2_3_4 | 5'-AGTTCCAGCG (A>G) CTGGACTCCG-3' | D145G | Substitution |
4 | 2_3_4 | 5'-AGCGACTGGA (C>T) TCCGCAGCCC-3' | T147I | Substitution |
5 | 2_3_4 | 5'-CAGCCCCAGA (T>A) CGGTCAGAAT-3' | I152N | Substitution |
6 | 2_3_4 | 5'-AAGCCGTTGT (G>T) AGCTCAGGTG-3' | Â | Intron |
7 | 2_3_4 | 5'-CCCGAGACCA (T>C) ACTCCCACCC-3' | I179T | Substitution |
8 | 2_3_4 | 5'-CTGTGGCAGA (T>C) CATCATCAAC-3' | I192T | Substitution |
9 | 2_3_4 | 5'-ATCATGGTTC (T>C) GACCGGTGCA-3' | L227P | Substitution |
10 | 2_3_4 | 5'-CGGTGCAGGT (G>T) TAGGTGTTTC-3' | Â | Splice |
11 | 2_3_4 | 5'-TAAAGAAACG (G>A) TAAACACCGG-3' | Â | Intron |
12 | 2_3_4 | 5'-CGGCTTGCTG (T>C) CGACTTTCCC-3' | V253A | Substitution |
13 | 5_6 | 5'-AACATCGACA (C>A) GCTGGAACAA-3' | T317K | Substitution |
14 | 5_6 | 5'-TGCGACGGCT (T>C) CCTGTCTCGT-3' | S338P | Substitution |
15 | 5_6 | 5'-CGTTTGTAAA (C>A) ACAAAGTGGA-3' | H344N | Substitution |
Blm | ||||
1 | 5_6 | 5'-AGCAGTAGGG (C>T) AATCTGTGTG-3' | A477V | Substitution |
2 | 5_6 | 5'-TGTGACTCTC (T>G) ATCAACTCCC-3' | L489R | Substitution |
3 | 5_6 | 5'-ACTTCTAAAA (C>T) AACCTTGTTT-3' | Q497X | Truncation |
4 | 5_6 | 5'-TTTCTCAGAG (A>G) GCACAAGTCG-3' | S503G | Substitution |
5 | 7 | 5'-TATTTTCTAT (C>T) TTCATTCAGA-3' | Â | Intron |
6 | 7 | 5'-CTTGATGCCC (A>G) CAGGTTGGTG-3' | T670A | Substitution |
Parkin ( Park2 ) | ||||
1 | 9_10_11 | 5'-ATGCACGGTA (C>G) CAGCAATATG-3' | Y314X | Truncation |
2 | 9_10_11 | 5'-GACTCATGTG (T>C) CCGGCACCTG-3' | C331C | Silent |
3 | 9_10_11 | 5'-AGGGTGGAGT (G>T) TGAGAGACAG-3' | C351F | Substitution |
4 | 9_10_11 | 5'-GCTGTGGCTT (T>A) GTCTTCTGTA-3' | F359L | Substitution |
5 | 9_10_11 | 5'-TTTTGTGATG (A>T) CATTGCCGTG-3' | Â | Intron |
6 | 9_10_11 | 5'-GTCTTATTCA (G>A) GAGATGACCA-3' | Q410Q | Silent |
7 | 9_10_11 | 5'-TCTCCACCTG (C>T) AGGTGGCTGC-3' | Â | Intron |
8 | 9_10_11 | 5'-TGCACATGCA (T>C) TGTGCTCTGT-3' | H433H | Silent |
9 | 9_10_11 | 5'-AGGGAGTGCA (T>A) GGGAAACCAC-3' | M454K | Substitution |