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Table 2 Medaka mutant library* screening statistics

From: Generation of medaka gene knockout models by target-selected mutagenesis

Gene Exons Exons screened Amplicons Base-pairs screened Exonic Intronic Total Mutation rate
      Stop Missense Silent Intron Splice   
Blm 23 2 2 3,129,006 1 4 0 1 0 6 1/521,501
p53 11 3 1 1,854,603 2 3 0 1 1 7 1/264,943
Sirt1 9 5 2 5,767,496 0 12 0 2 1 15 1/384,500
Dcr-1 27 7 4 7,879,290 0 16 4 7 0 27 1/291,826
Parkin 11 3 1 3,461,661 1 3 3 2 0 9 1/384,629
Total 81 20 10 22,092,056 4 38 7 13 2 64 1/345,188
  1. *The mutant library consists of 5,771 cryopreserved male progeny from ENU-mutagenized fish. Due to the compact medaka genome architecture, multiple exons can often be amplified and sequenced from a single amplicon. Determined by counting all bases in the resequencing reads that were read with phred quality >20.