Generation of medaka gene knockout models by target-selected mutagenesis

Table 2 Medaka mutant library* screening statistics

Gene	Exons	Exons screened	Amplicons^†	Base-pairs screened^‡	Exonic			Intronic		Total	Mutation rate
					Stop	Missense	Silent	Intron	Splice
Blm	23	2	2	3,129,006	1	4	0	1	0	6	1/521,501
p53	11	3	1	1,854,603	2	3	0	1	1	7	1/264,943
Sirt1	9	5	2	5,767,496	0	12	0	2	1	15	1/384,500
Dcr-1	27	7	4	7,879,290	0	16	4	7	0	27	1/291,826
Parkin	11	3	1	3,461,661	1	3	3	2	0	9	1/384,629
Total	81	20	10	22,092,056	4	38	7	13	2	64	1/345,188

*The mutant library consists of 5,771 cryopreserved male progeny from ENU-mutagenized fish. ^†Due to the compact medaka genome architecture, multiple exons can often be amplified and sequenced from a single amplicon. ^‡Determined by counting all bases in the resequencing reads that were read with phred quality >20.

ISSN: 1474-760X