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Figure 4 | Genome Biology

Figure 4

From: Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors

Figure 4

U2AF65 associates with fully spliced mRNAs. Agarose gel electrophoresis of RT-PCR reactions designed to check the splicing status of mRNAs present in control (Mock) and anti-U2AF65 (U2AF) RNA-immunoprecipitations. For each target, the transcript exon/intron structure is represented to scale and primer localization and expected size of fully spliced products are indicated. IP, immunoprecipitation; RT-PCR, reverse transcription polymerase chain reaction; U2AF, U2 small nuclear RNP auxiliary factor.

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