|
Case
|
Expected errors Plimit = 0.9
|
Expected errors Plimit = 0.95
|
Expected errors Plimit = 0.99
|
|---|
|
MS/MS Pi ≥ Plimit
|
0.00915 (9%)
|
0.00517 (5%)
|
0.00137 (1%)
|
|---|
|
MS/MS Pi ≥ Plimit, Npeptide observed > 1
|
0.00884 (9%)
|
0.00506 (5%)
|
0.00136 (1%)
|
|
Consensus peptide best Pi, Pi ≥ Plimit
|
0.01027 (10%)
|
0.00510 (5%)
|
0.00120 (1%)
|
|
Consensus peptide best Pi, Pi ≥ Plimit, Npeptide observed >1
|
0.00272 (3%)
|
0.00215 (2%)
|
0.00078 (1%)
|
- Expected errors in the Saccharomyces PeptideAtlas are calculated for four cases and lower probability limits (Plimit = 0.9, 0.95, 0.99): the assigned probability P of each MS/MS is used for all Pi ≥ Plimit; the assigned probability P of each MS/MS is used for all Pi ≥ Plimit where the associated peptide has been seen in the S. cerevisiae PeptideAtlas more than once'; the best probability for each unique peptide sequence is used for all Pi ≥ Plimit; the best probability for each unique peptide sequence is used for all Pi ≥ Plimit when the peptide has been seen in the S. cerevisiae PeptideAtlas more than once.
