Conservation of large most conserved elements across species. (a) The red data points show conservation in the LMCEs (defined as MCEs ≥360 base pairs) plotted as pair-wise identities across all species in the underlying seven-way vertebrate whole-genome alignments with human. The blue lines indicate the mean and standard errors of the mean for each species relative to human. Orthologous species include chimpanzee (Pan troglodytes [pt]), dog (Canis familiaris [cf]), mouse (Mus musculus [mm]), rat (Rattus norvegicus [rn]), chicken (Gallus gallus [gg]), zebra fish (Danio renio [dr]), and puffer fish (Fugu rubripes [fr]). (b) Species are plotted against the proportion of total LMCE length showing homologous sequence devoid of insertions or deletions in the underlying whole-genome multiple alignment. LMCE, large most conserved element.