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Table 2 P[GT1]-element insertion lines with transposon insertions at genes with ethanol-dependent altered transcriptional regulation

From: Transcriptional response to alcohol exposure in Drosophila melanogaster

Gene symbol

P-element insertion site

Biological process

Canton S* background

Mean elution time (min)† (1st exposure)

Time shift (between 1st and 2nd exposures)

Slow down-regulation

   Fkbp13

In 1st intron

Protein folding

A

6.7 ± 0.25

2 min‡ ↑

   Sir2

410 bp in exon 1

Regulation of transcription, DNA-dependent; chromatin silencing

B

5.0 ± 0.2

2 min§ ↓

   BubblegumH

In 1st intron

Fatty acid metabolism

C

6.2 ± 0.21

0.5 min (NS) ↓

Acute up-regulation

   CG1516H

125 bp upstream of transcription initiation site of the longest transcript

Pyruvate metabolism; fatty acid biosynthesis

E

4.2 ± 0.12

1 min (NS) ↑

Transient up-regulation

   Thor

431 bp upstream of transcription initiation site

Negative regulation of translational initiation; response to stress; defense response

E

7.5 ± 0.11; R

2.5 min§ ↑

   Pyruvate dehydrogenase kinaseH

47 bp in exon 1

Pyruvate metabolism; tricarboxylic acid cycle; regulation of phosphate metabolism

F

7.4 ± 0.30; R

1 min‡ ↑

Slow up-regulation

   v(2)k05816H

281 bp upstream of transcription initiation site

Fatty acid biosynthesis

A

6.1 ± 0.25

1 min (NS) ↑

   elbow BH

888 bp upstream of transcription initiation site

Tracheal system development

A

6.8 ± 0.26

0.5 min (NS) ↓

   Hormone receptor-like in 38

1,428 kb upstream of transcription initiation site

Regulation of transcription from RNA polymerase II promoter; intracellular signaling cascade

A

5.8 ± 0.22; S

1 min (NS) ↓

   nuclear falloutH

In 4th intron

Actin cytoskeleton reorganization; microtubule-based process

B

6.6 ± 0.30

1 min (NS) ↓

   CG12505

136 bp upstream of transcription initiation site

Unknown

B

4.8 ± 0.18

1.5 min‡ ↓

   tramtrack

219 bp in exon 1 of the longest transcript

Regulation of transcription from RNA polymerase II promoter; peripheral nervous system development

B

5.8 ± 0.20

1 min§ ↑

   CG9086H

50 bp in exon 1

Protein ubiquitination

B

6.1 ± 0.18

1.5 min§ ↓

   CG6767

In 1st intron

Nucleotide biosynthesis, metabolism

B

7.0 ± 0.16; R

0.5 min (NS) ↑

   CG32434

36 bp upstream of transcription initiation site

Unknown

B

6.1 ± 0.26

2 min§ ↓

   frizzled 2H

147 bp in exon 1

Signal transduction; G-protein coupled receptor protein signaling pathway

E

5.9 ± 0.13; R

2 min§ ↑

   Malic enzymeH

In 1st intron

Malate metabolism; tricarboxylic acid cycle

E

6.4 ± 0.15; R

6 min¶ ↑

   CG9238

73 bp in exon 1

Glycogen metabolism

F

7.6 ± 0.12; R

3.5 min§ ↑

   spalt majorH

154 bp upstream of transcription initiation site

Tracheal system development; sensory organ development; negative regulation of transcription

F

6.9 ± 0.18; R

0.5 min (NS) ↓

   lola

In 1st intron

Transmission of nerve impulse; regulation of transcription from RNA polymerase II promoter

F

6.1 ± 0.11; R

3 min§ ↑

  1. *See Additional data file 5 for mean elution times of P-element free Canton S genetic backgrounds A, B, C, E and F. †S and R designate P-element insert lines that are significantly more sensitive or more resistant than the co-isogenic control, respectively (one-way ANOVA). An H after a gene symbol indicates genes that have human orthologues. Up and down arrows designate increase or decrease, respectively, in the time shift between the first and second ethanol exposure compared with the co-isogenic control (Student's t-test). ‡p < 0.05, §p < 0.01, ¶p < 0.001. bp, base-pairs; NS, not significant.