Figure 3

ChIP CHIP results on the two neocentromere cases. (a) Ideogrammatic representation of the neocentric chromosomes found in the two independent neocentromere cases. From left to right: the invdup13q14 chromosome with a neocentromere in band 13q21; the ring chromosome derived from band 13q21 to 13q22; a normal chromosome 13 for comparison. At the far right, the region 13q21.3 is expanded to show the relative position of BAC clones H2 to H9, along with the human neocentromeres and evolutionarily new centromeres of OWM and pig (see also Figure 2b). (b) ChIP on a CHIP analysis of the invdup13q14 chromosome using antibodies to CENP-A. (3) ChIP on a CHIP analysis of the ring13q21 chromosome using antibodies to CENP-C. For (b and c), the microarray was hybridized simultaneously with Cy-5 labeled CENP ChIP DNA (red) and Cy3 labeled input chromatin DNA (green). The scale normalized mean Log2 Cy-5:Cy-3 ratios and standard error for each BAC are shown plotted on the y-axis for three independent ChIP experiments. Alpha satellite DNA was included in the microarray as a positive control (far right). (3) Position of 107 contiguous BACs spanning 13q21 to 13q22 is shown across the x-axis. Positions of probes H7, H8, H9 and I (Table 1) are shown. Bottom: blow up of BACs that are positive for CENP proteins. Thin lines represent regions of BAC overlap. Scale in Mbp according to UCSC genome coordinates hg17 [Bioinformatics, #9821].