Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial

Table 1 PA14 and PAO1 genome comparisons

	PA14	PAO1
Genome size	6,537,648	6,264,403
GC content	66.3 ± 4.3%^a	66.6 ± 3.9%^a
Total number of genes	5973	5651
Class 1 genes^b	8.6%	9.0%
Class 2 genes^b	18.0%	20.0%
Class 3 genes^b	44.5%	26.8%
Class 4 genes^b	28.9%	44.2%
Number of strain-specific regions^c	58	54
Total DNA in strain-specific regions	541,215 (8.3%)	261,426 (4.2%)
GC content (strain-specific regions)	59.60%	59.80%
Total number of genes (strain-specific regions)	478	234
Class 1 genes^b	3.3%	9.8%
Class 2 genes^b	4.0%	3.0%
Class 3 genes^b	29.7%	30.8%
Class 4 genes^b	63.0%	56.4%

^aStandard deviation was calculated using a sliding 1 kb window (see Additional data file 1). ^bClasses 1-4 refer to the confidence rating assigned to the predicted gene function, as previously described [7,22]. Class 1 genes are those whose function has been experimentally validated in P. aeruginosa. Class 2 genes are highly similar to genes whose functions have been validated in another organism. Class 3 genes have hypothesized functions based on limited similarity to other genes or structural/functional domains. Class 4 genes are open reading frames (ORFs) of unknown function. ^cStrain-specific regions with at least one ORF.

ISSN: 1474-760X