Figure 6

Verification of the predicted dBP of IDB1088375 exon 2. (a) Output from our prototype data set. The various field labels are as described in the legend to Figure 4. The potential BPS indicated in bold red and blue were mutagenized. (b) IDB1088375 exon 2 and flanking intron sequences were cloned in the EGFP exon trapping vector [21]. Mutants ΔBP-160/-166 and -81 contained the indicated mutations in potential branch points. Constructs were transfected into HeLa cells, and RNA analyzed by reverse transcriptase polymerase chain reaction. Splicing of exon 2 was reduced in ΔBP-160/-166, but not in -81.