Figure 5

Verification of the predicted dBP of GABBR1 exon 23. (a) Output for GABBR1 exon 23 from our prototype data set. The various field labels are as described in the legend to Figure 4. The two magenta colored Ts are sites of single nucleotide polymorphisms and can be T or C. The potential BPS indicated in bold red and blue were mutagenized. (b) GABBR1 exon 23 and flanking intron sequences were cloned in the EGFP exon trapping vector [21]. Mutants ΔBP-275 and -217 contained the indicated mutations in potential branch points. Constructs were transfected into HeLa cells, and RNA analyzed by reverse transcriptase polymerase chain reaction. Splicing of exon 23 was abolished in ΔBP-275.