Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae

Table 3 Final concentration of elements in growth medium

Element	Form added	Supplemented concentration	Final concentration
Calcium	CaCl₂	1 mM	1.2 mM
Cobalt	CoCl₂	5 μM	5.1 μM
Copper	CuCl₂	100 μM	110 μM
Iron	-	-	19 μM
Magnesium	-	-	490 μM
Manganese	MnCl₂	10 μM	11 μM
Nickel	NiCl₂	125 μM	160 μM
Potassium	-	-	12 mM
Phosphorus	-	-	5.7 mM
Selenium	Na₂SeO₃	75 μM	75 μM
Sodium	-	-	16 mM
Sulfur	-	-	5.4 mM
Zinc	ZnCl₂	100 μM	160 μM

Calcium, cobalt, copper, manganese, nickel, selenium and zinc were added to rich YPD medium (1% yeast extract, 2% peptone, 2% glucose) to facilitate their detection in cells by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). These supplemented levels did not exceed 10% of the minimal growth inhibitory concentration determined for this strain (data not shown). The final concentration of these elements in the growth medium measured by ICP-AES is also shown and represents the supplemented levels plus those present in the YPD medium alone. The same lots of all medium components (such as yeast extract, peptone) were used throughout this study to maintain consistent growth conditions. Boron was added as H₂BO₃ at 181 μM and molybdenum was added as NaMoO₄ at 10 μM. Despite this supplementation, levels of these two minerals accumulated by cells remained below the level of detection. -, not supplemented.

ISSN: 1474-760X