Figure 5

Comparison of Yfr2, Yfr3, Yfr4 and Yfr5 from MED4. (a) Sequence comparison of the yfr2 through yfr5 coding regions of MED4. Transcriptional initiation sites (TIS) and the deduced -10 elements are indicated. The location of specific oligonucleotide probes y2aM, y3aM, y4aM and y5aM used in Figure 5b and in 5' RACE and of the y_gen consensus probe used in Figure 4 is indicated by the lines with black diamonds on the ends on top of the alignment. (b) Signals for the four individual non-coding RNAs (ncRNAs) were detected in Northern blots using probes y2aM, y3aM, y4aM and y5aM. These probes have a minimum of five mismatches to their non-target ncRNAs, making cross-hybridizations impossible. The numbers indicate transcript lengths in nucleotides. (c) Prediction of secondary structure of MED4 Yfr2 by MFOLD [59].