Figure 1

Design and reproducibility of microarrays. (a) Examples of microarrays: three membranes were used for each biological sample; arrays for Pu.1-/- cells, BMMC and hippocampus are shown. On the left, ethidium bromide staining of total RNA run on a denaturing gel for RNA quantification and quality control. (b) Plot of the standard deviation over replicates versus the mean of each replicate. The red line is a lowest fit to the distribution and the blue dotted line is twice the value of the red one. Points below the blue curve are considered good replicates; those above it are filtered out as too noisy. For this dataset, 86% of the spots were considered as good. BMMC, bone marrow-derived mast cells.