Figure 3
From: The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles
Growth of RasGEF mutants in axenic suspension. Vegetative AX3 and gef null cells were grown in axenic medium in shaken flasks. At the indicated times, samples were taken and counted in duplicate using a hemocytometer. Mutants in gefK and gefL were observed on different occasions but were similarly comparable to the parental strain.