Predicted Hsf-binding sequences in the 1-kb region upstream of Hsf-binding genes. (a) Plot of the distribution of the number of predicted sites as a proportion of the population of anti-Hsf-enriched (Heat shock) or non-enriched (Control). (b) The relative position of predicted Hsf sites for each of the genes containing eight or more sites. The annotated gene start is on the right. Red triangles, perfect match; purple, one mismatch; light blue, two mismatches. Gray boxes represent the known HSEs upstream of Hsp23, Hsp26 and Hsp27.