Validation and refinement of gene-regulatory pathways on a network of physical interactions

Table 2 Top-ranking knock-out experiments proposed for model discrimination

Gene	Function	Score	Downstream genes	Rank	Model
HHF1	Histone	52.1429	74	1	2
SOK2*	Regulator for meiosis and PKA pathway	45.0279	64	2	1
CKA1	Protein kinase of cell cycle	45.0075	64	3	5
A2	Mating response	40.9023	58	4	4
YAP6*	Stress response regulator	35.1652	50	5	1, 3
NRG1	Regulator of glucose dependent genes	31.6501	45	6	3
FKH1	Regulator of cell cycle	29.1194	41	7	2
FKH2	Regulator of cell cycle	26.7131	38	8	7
SLT2	Protein kinase of cell wall integrity pathway	23.4727	31	9	8
MSN4*	Regulator of stress response	21.8224	31	10	1
HAP4*	Regulator of cellular respiration	6.3310	9	34	1

Each proposed target gene is reported, along with its function, mutual information score, rank, and the model(s) it informs. All target genes are non-lethal in rich media. *Gene knockouts selected in this study.

ISSN: 1474-760X