From: Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray
Yeast intronic/intergenic control transcript | Vector name ATCC number GenBank Accession | Insert size (bp) | Copies spiked/5 μg total RNA | Forward/reverse qPCR oligo sequence | Optimal concentration | Amplicon | Intron spanned? | |
---|---|---|---|---|---|---|---|---|
 |  |  |  |  |  | Size | Tm |  |
YPL075W_16_412249_415357_INTRON_9_759 | pNIAysic-1 MBA-201 DQ023287 | 630 | 1.00E+04 | 5'-CCTACTTGATAAAGCCACATACCTCTACCTCTTCTATTAG-3' 5'-TTGCGTTACTCTATTAATAATCCATAGTTGGAAC-3' | 300 nM 50 nM | 134 bp | 73.4°C | No |
YPL081W_16_404945_406039_INTRON_8_508 | pNIAysic-2 MBA-202 DQ023288 | 400 | 1.00E+05 | 5'-CGACACTTCAGGTAAAGCGTTCCGAAGTAATTCAAC-3' 5'-TCTCAAACCTAACACATTTCTGTATTAAGCCTAG-3' | 300 nM 300 nM | 129 bp | 75.8°C | No |
NOT:D_1493031-1494574_553-1543 | pNIAysic-3 MBA-203 DQ023289 | 997 | 1.00E+06 | 5'-TTACCATTCACTCCATGATGTCGTACCTGTTACACTAC-3' 5'-CGGTACATGTTATTACCAGAAAAAGATGTATATCC-3' | 300 nM 300 nM | 145 bp | 79.8°C | No |
YER133W_5_432491_433954_INTRON_178_702 | pNIAysic-4 MBA-204 DQ023290 | 428 | 1.00E+07 | 5'-GTCGAGATAGCCGAGATAATGTGTGTG-3' 5'-GCAAGGGGGATTTTTCTGAATATGG-3' | 300 nM 300 nM | 136 bp | 76.5°C | No |
YNL162W_14_331319_332151_INTRON_5_516 | pNIAysic-5 MBA-205 DQ023291 | 367 | 1.00E+08 | 5'-TGCAGCAACAGAGTATCATATGCATGG-3' 5'-CACTGCACAATCTGAAGATAGCGAGG-3' | 300 nM 300 nM | 145 bp | 77.7°C | No |
YNL302C_14_62942_61957_INTRON_21_571 | pNIAysic-6 MBA-206 DQ023292 | 416 | 1.00E+09 | 5'-ATTTCCCATTACCTGATAAATTGAAGTTCATC-3' 5'-TTTGTATAGTTGGCTCAAAATATTCTCTCCAC-3' | 900 nM 300 nM | 100 bp | 73.8°C | No |
YBL087C_2_60732_59815_INTRON_43_546 | pNIAysic-7 MBA-207 DQ023293 | 436 | 1.00E+010 | 5'-GCAGATGAAGTGATACCTGTCAATATTCATG-3' 5'-AGAAATAACATTTCGATGGTTATCCATTAGTATG-3' | 300 nM 300 nM | 128 bp | 76.2°C | No |