Figure 3

Exogenous control and endogenous transcript amplification rates are closely matched over seven orders of magnitude. Transcript abundance of each spike-in control transcript was measured by qPCR before and after linear amplification labeling, and compared to amounts of the exogenous transcript Dnchc1. After amplification, individual ratios of each control transcript to the endogenous transcript were within 3.5-fold (average = 1.98-fold) of those prior to amplification. Blue diamonds = log₁₀[ratio mean control/Dnchc1 transcripts] of three E12.5 embryo and three E12.5 placenta samples before amplification. Red boxes, green triangles = log₁₀[ratio mean control/Dnchc1 transcripts] for the same samples after amplification, using yield versus input (red boxes) or the increase in Dnchc1 transcripts as measured by qPCR (green triangles) to calculate the fraction of the original sample represented by each qPCR well.