Figure 4

Tissue-specific gene-expression patterns measured using the rhesus macaque oligonucleotide microarray. To evaluate whether arrayed oligonucleotides were binding target sequences, microarrays were hybridized with probes derived from RNA isolated from spleen, brain, or placenta. Probes were paired in different combinations as indicated: (a) spleen vs brain; (b) brain vs placenta; (c) spleen vs placenta. Oligonucleotides that detected a difference in gene expression of twofold or more (P ≤ 0.01) between two tissues are indicated as colored points and are displayed across the x-axis to facilitate visualization. The y-axis represents the log ratio for differentially expressed genes in each tissue comparison. Points corresponding to oligonucleotides derived from spleen ESTs are colored blue, those from brain are magenta, and those from placenta are orange. Thus, in the comparison depicted in (a), genes more highly expressed in the spleen are indicated by points in the upper portion of the panel (and are predominantly sequences derived from the spleen cDNA library) and genes more highly expressed in the brain are indicated by points in the lower portion on the panel (and are predominantly sequences derived from the brain cDNA library). Plots were prepared using Spotfire.