Absolute metabolic fluxes in the 37 flexible mutants as a function of glucose uptake rate or selected intracellular fluxes. (a-f) Glucose uptake rate; (g,h) selected intracellular fluxes. The linear regression of the distribution and the 99% prediction interval are indicated by the solid and dashed lines, respectively. Mutants with significant changes in the carbon-flux distribution are indicated. The reference strain is indicated by an open circle. Extreme flux patterns were verified in 30-ml shake flask cultures (data not shown).