Skip to main content

Advertisement

Table 1 Fold change in gene expression after 4 h exposure to NCS as measured by microarrays and by quantitative real-time RT-PCR

From: Dissection of a DNA-damage-induced transcriptional network using a combination of microarrays, RNA interference and computational promoter analysis

Gene Affy_ID Fold induction microarray Fold induction RT-PCR
   C LacZ Rel-A (NFκB) p53 ATM C Rel-A (NFκB) p53 ATM
(a) Known direct targets of NFκB
TNFAIP3 202644_s_at 8.28 5.34 1.15 3.02 1.19 9.5 1.1 9.5 0.9
RELB 205205_at 3.7 2.89 0.82 2.95 0.91 15.7 6.0 21.3 2.5
TNFRSF9 207536_s_at 4.01 3.5 1.1 2.08 1.21 14.3 3.5 11.0 1.4
NFKBIA 201502_s_at 4.61 5.4 1.26 2.67 1.02 4.2 1.7 4.5 1.2
CD83 204440_at 3.46 2.99 1.0 1.73 1.06 6.5 1.0 5.7 1.3
IER3 201631_s_at 4.44 5.12 1.43 2.35 1.44 6.6 1.8 3.4 1.8
(b) Known direct targets of p53
ATF3 202672_s_at 3.44 3.74 7.03 1.54 1.47 5.2 5.9 1.6 1.6
EGR1 201694_s_at 2.78 1.77 6.77 1.04 1.02 4.4 13.4 0.7 2.4
JUN* 213281_at 2.01 1.45 2.71 1.36 1.25 6.6 3.9 0.64 2.5
FOS 209189_at 1.72 1.42 2.22 1.07 1.22 3.4 13.1 3.4 1.9
ETR101* 202081_at 1.97 2 2.6 1.06 1.13 2.0 3.0 1.4 1.4
GADD45A 203725_at 2.36 2.07 2.00 1.07 1.22 1.8 2.3 1.8 1.3
DUSP1 201041_s_at 2.06 2.57 3.45 1.11 1.22 2.2 4.5 2.0 1.9
  1. *These genes are not reported as direct targets of p53 but are known to be functionally related to p53.