Alignment of GPCR-like proteins. Domains conserved in (a) PTH11-, (b) cAMP-, (c) STM1- and (d) mPR-related classes are shown. Representative sequences from each class were aligned using T_Coffee . The alignment was analyzed using GenDoc. We used the default setting using the conservative shading mode with similarity groups enabled. Black and the dark and light gray represent 80% or greater conserved, 60% or greater conserved, and less than 60% conserved, respectively. Conservative substitutions were counted as a single residue type. The GenBank or Swiss-Prot (SP) accession numbers or the accession numbers of the predicted proteins in the M. grisea or N. crassa genome databases are indicted on the left [21, 42]. The boundaries of each sequence used in the alignment are indicated on the right.