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Figure 2 | Genome Biology

Figure 2

From: Connecting the dots in Huntington's disease with protein interaction networks

Figure 2

Schematic representations of library and matrix yeast two-hybrid screens. (a) A model of the yeast two-hybrid system. The DNA-binding domain (BD) and transcriptional activation domain (AD) from a transcription factor are independently fused with candidate interacting proteins (the bait and prey, respectively). If the bait and prey proteins interact (curved line) within a cell expressing both fusions, the resulting functional transcription factor can bind the promoter of a reporter gene and activate its transcription by interacting with the general transcription machinery (G). (b) A library yeast two-hybrid screen. A collection of preys are screened with a bait of interest by transforming yeast cells with plasmids encoding the constructs in order to isolate its interaction partners. (c) A matrix yeast two-hybrid screen used to generate a protein-protein interaction network. Several baits and preys are arrayed in 96-well microtiter plates and the fusion proteins are brought together by mating. Diploids containing both bait and prey are isolated on selective plates and protein-protein interactions are ascertained by expression of the reporter gene. The dark squares indicate an interaction between the bait given at the end of the row and the prey indicated at the top of the column.

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