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Table 1 The number of clones and assigned fold change for each pool of PCR products

From: Preferred analysis methods for Affymetrix GeneChips revealed by a wholly defined control dataset

Pool number Number of clones Number of assigned Affymetrix probe sets Assigned fold change (S vs C) Amount of RNA added to each C chip (μg) Amount of RNA added to S chip (μg)
1 87 84 1.2 0.47 0.56
2 141 143 2 0.43 0.85
3 85 83 1.5 0.35 0.52
4 180 185 2.5 0.73 1.82
5 90 89 1.2 0.29 0.35
6 88 96 3 0.65 1.94
7 186 188 3.5 0.76 2.67
8 90 95 1.5 0.44 0.67
9 180 190 4 0.78 3.11
10 183 191 1.7 0.48 0.81
13 391 385 1 0.37 0.37
14 369 355 1 1.23 1.23
15 394 404 1 0.40 0.40
16 452 453 1 0.57 0.57
17 419 434 1 0.44 0.44
18 372 407 1 0.31 0.31
19 163 191 1 0.27 0.27
  1. Also depicted is the total amount of cRNA for each pool that was placed on each chip, and the number of Affymetrix probe sets that are assigned to each pool. There were 10,131 probe sets not assigned to any spiked-in clone (called empty). Pools 11 and 12 were not included in this dataset.