Table 1 Concentration and doses of cytokines/soluble factors used for stimulation of monocytes
Cytokine | Concentration in vitro | Source |
|---|---|---|
Aldara | 3 μM | 3M Pharmaceuticals |
BCA-1 | 10 ng/ml | Peprotech |
CD40 L | 500 ng/ml | Peprotech |
FLT-3 ligand | 100 ng/ml | Peprotech |
GM-CSF | 1,000 IU/ml | Peprotech |
IFN-γ | 1,000 U/ml | Biogen |
IFN-α2 | 1,000 U/ml | Peprotech |
IFN-α2b (α2, α2b,) | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αA (2a) | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αI | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αB2 | 1,000 U/ml | PBL Biomedical Laboratorios |
IFN-α4b | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αC | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αF | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αG | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αH2 | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αJ1 | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αK | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-αWA | 1,000 U/ml | PBL Biomedical Laboratories |
IFN-β | 1,000 U/ml | PBL Biomedical Laboratories |
IL-1α | 10 ng/ml | National Cancer Institute (NCI) Biological Research Branch |
IL-1β | 10 ng/ml | NCI Biological Research Branch |
IL-2 | 6,000 IU/ml | Chiron |
IL-3 | 15 ng/ml | NCI Biological Research Branch |
IL-4 | 1,000 IU/ml | Peprotech |
IL-5 | 10 ng/ml | Peprotech |
IL-6 | 100 ng/ml | Peprotech |
IL-8 | 100 ng/ml | Peprotech |
IL-10 | 10 ng/ml | Peprotech |
IL-12 | 10 ng/ml | Peprotech |
IL-13 | 20 ng/ml | Peprotech |
IL-15 | 20 ng/ml | Peprotech |
LPS only | 5 μg/ml | Sigma Aldrich |
MIP-1α (CCL3) | 100 ng/ml | Peprotech |
MIP-4 (PARC, CCl18) | 100 ng/ml | Peprotech |
MIP-1β (CCL4) | 100 ng/ml | Peprotech |
RANTES (CCL5) | 100 ng/ml | Peprotech |
TARC (CCL17) | 100 ng/ml | Peprotech |
TGF-α | 10 ng/ml | Peprotech |
TNF-β | 20 ng/ml | Peprotech |
TGF-β | 5 ng/ml | Peprotech |
TNF-α | 100 ng/ml | Peprotech |
VEGF | 10 ng/ml | R&D Systems |