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Table 1 Concentration and doses of cytokines/soluble factors used for stimulation of monocytes

From: Polarized monocyte response to cytokine stimulation

Cytokine Concentration in vitro Source
Aldara 3 μM 3M Pharmaceuticals
BCA-1 10 ng/ml Peprotech
CD40 L 500 ng/ml Peprotech
FLT-3 ligand 100 ng/ml Peprotech
GM-CSF 1,000 IU/ml Peprotech
IFN-γ 1,000 U/ml Biogen
IFN-α2 1,000 U/ml Peprotech
IFN-α2b (α2, α2b,) 1,000 U/ml PBL Biomedical Laboratories
IFN-αA (2a) 1,000 U/ml PBL Biomedical Laboratories
IFN-αI 1,000 U/ml PBL Biomedical Laboratories
IFN-αB2 1,000 U/ml PBL Biomedical Laboratorios
IFN-α4b 1,000 U/ml PBL Biomedical Laboratories
IFN-αC 1,000 U/ml PBL Biomedical Laboratories
IFN-αF 1,000 U/ml PBL Biomedical Laboratories
IFN-αG 1,000 U/ml PBL Biomedical Laboratories
IFN-αH2 1,000 U/ml PBL Biomedical Laboratories
IFN-αJ1 1,000 U/ml PBL Biomedical Laboratories
IFN-αK 1,000 U/ml PBL Biomedical Laboratories
IFN-αWA 1,000 U/ml PBL Biomedical Laboratories
IFN-β 1,000 U/ml PBL Biomedical Laboratories
IL-1α 10 ng/ml National Cancer Institute (NCI) Biological Research Branch
IL-1β 10 ng/ml NCI Biological Research Branch
IL-2 6,000 IU/ml Chiron
IL-3 15 ng/ml NCI Biological Research Branch
IL-4 1,000 IU/ml Peprotech
IL-5 10 ng/ml Peprotech
IL-6 100 ng/ml Peprotech
IL-8 100 ng/ml Peprotech
IL-10 10 ng/ml Peprotech
IL-12 10 ng/ml Peprotech
IL-13 20 ng/ml Peprotech
IL-15 20 ng/ml Peprotech
LPS only 5 μg/ml Sigma Aldrich
MIP-1α (CCL3) 100 ng/ml Peprotech
MIP-4 (PARC, CCl18) 100 ng/ml Peprotech
MIP-1β (CCL4) 100 ng/ml Peprotech
RANTES (CCL5) 100 ng/ml Peprotech
TARC (CCL17) 100 ng/ml Peprotech
TGF-α 10 ng/ml Peprotech
TNF-β 20 ng/ml Peprotech
TGF-β 5 ng/ml Peprotech
TNF-α 100 ng/ml Peprotech
VEGF 10 ng/ml R&D Systems