Effect of cytokines on the expression of genes dependent on NFκB activation. (a) One hundred and twenty-one genes associated with the downstream effects of NFκB activation were selected from the unfiltered 17K gene dataset and reclustered without changing the cytokine treatment grouping as per Figure 2. The genes most significantly different in expression between the two classes are listed on the right. (b) All samples were reclustered (dendrogram) using the 121 genes described in (a). Color coding is identical to that in Figure 2. (c) The histogram depicts detection of the free NFκB subunit p50 in four consecutive experiments. p = p2 value, NS, nonsignificant. Blue bars, MPs stimulated with LPS (1 h) + alternative cytokines (additional 30 min stimulation); red bars, classical cytokines; yellow bar, MPs stimulated with LPS alone; gray bar, unstimulated MP control; purple bar, TNF-α alternative II class of cytokines.