Experimental validation of bioPIXIE prediction for the biological role of YPL017C. bioPIXIE was used to predict previously uncharacterized genes likely to participate in processes related to chromosomal segregation (data for YPL017C shown). Yeast cells were fixed, stained, and photographed using differential interference contrast imaging and 4'-6-diamidino-2-phenylindole (DAPI) staining. When compared with wild-type cells, populations of cells lacking YPL017C have a higher proportion of large-budded cells with a single nucleus at the bud neck (75% compared to 22% in wild type, Fisher exact test P value of 5 × 10-9). Large budding cells are indicated by arrows. This morphology and failure of nuclear separation are analogous to that of ctf4Δ mutants , supporting the hypothesis that YPL017C, like CTF4, is involved in chromosome segregation. See Figure S8 in  for experimental verification of YPL077C and YPL144W.