Figure 7

Response of AQY1 to osmotic stress. Cells were either grown in YPD (filled circles) or shocked by sorbitol addition for 30 minutes (open circles). Sucrose gradient centrifugation was performed and analyzed as described in Figure 2. The top of the gradient is to the left and the position of the 80S monosome is marked with the arrow. The 5' termini of the AQY1 transcript before and after osmotic stress are shown in the inset. Rapid amplification of cDNA ends (RACE) was carried out as described in the text (see Materials and methods), using as templates total RNA isolated from cells either grown in YPD (-) or osmotically shocked (+). The image contains the polymerase chain reaction products from the second RACE amplification step after separation by electrophoresis in 2% agarose gel. Lane 'M' contains a 100-basepair ladder.