Figure 6

Changes in ribosome loading in response to nitrogen starvation. mRNA levels across sucrose gradient from growing cells (open circles) and from cells nitrogen starved for 30 minutes (filled circles) for (a) DAL5, (b) UGA1 and (c) GCN4. RNA was prepared and analyzed as described in Figure 2. The top of the gradient is to the left and the position of the 80S monosome is marked with the arrow. The experiments shown in (a) and (b)were performed with strain LL1 (Δgcn2 ; described in Materials and methods) and the experiment in panel c was conducted with the wild-type strain.