Figure 5

Translational responses to nitrogen starvation. Sucrose gradient centrifugation was performed and analyzed as described in Figure 2. The A₂₅₄ profiles are shown of sucrose gradients with extracts from either (a) growing cells or (b) starved cells loaded onto gradients. The tops of the gradients and location of the 80S ribosome peak in panel a are indicated. (c) ASP1 mRNA levels across sucrose gradients from growing cells (filled circles) or cells nitrogen starved for 30 minutes (open squares). RNA was prepared and analyzed as described in Figure 2. The top of the gradient is to the left and the position of the 80S monosome is marked with the arrow. (d) ASP3 mRNA levels; cell extracts and symbols are as in (c).