Molecular processes during fat cell development revealed by gene expression profiling and functional annotation

Table 2 Transcription factors that could regulate co-expressed genes in each cluster

Binding factors	Over-represented cluster	CS	FE	Putative target genes	Genes in cluster with promoter in PromoSer database	Putative target genes of all clusters
RORα1	Cluster 1	0.0322	0.0203	10	10	240
ATF	Cluster 2	0.0466	0.0481	15	27	133
CRE-BP1	Cluster 2	0.0050	0.0050	19	27	153
HLF	Cluster 2	0.0436	0.0452	15	27	132
XBP-1	Cluster 2	0.0378	0.0476	4	27	17
AhR	Cluster 2	0.0287	0.0446	3	27	9
Tal-1β/E47	Cluster 3	0.0400	0.0427	9	15	123
v-Maf	Cluster 4	0.0432	0.0308	2	12	11
SREBP-1	Cluster 4	0.0494	0.0484	9	12	166
Tal-1β/ITF-2	Cluster 5	0.0145	0.0169	19	46	89
Pbx-1	Cluster 5	0.0323	0.0206	45	46	312
NRF-2	Cluster 5	0.0310	0.0252	41	46	270
Sox-5	Cluster 5	-	0.0490	40	46	268
VBP	Cluster 5	0.0345	0.0276	42	46	281
NF-κB (p65)	Cluster 6	0.0354	0.0333	13	17	182
CCAAT box	Cluster 6	0.0458	0.0287	17	17	288
AP-2	Cluster 6	0.0330	0.0268	15	17	226
E4BP4	Cluster 8	0.0230	0.0243	31	69	113
CCAAT	Cluster 8	0.0211	0.0304	5	69	7
VBP	Cluster 8	0.0242	0.196	62	69	281
GC box	Cluster 9	-	0.0450	44	48	289
RREB-1	Cluster 10	0.0388	0.0435	13	42	65
SRF	Cluster 10	0.0221	0.0255	16	42	81
GC box	Cluster 10	0.0450	0.0366	39	42	289
Poly A downstream element	Cluster 11	0.0335	0.0431	5	13	55
E2	Cluster 12	0.0459	-	14	47	65

Probabilities for over-representation (<0.05) of genes having a predicted transcription factor binding site relative to the total of all clusters. CS, one-sided χ² test; FE, one-sided Fisher's exact test.

ISSN: 1474-760X