Skip to main content

Advertisement

Table 10 Quantitative real-time PCR results for selected non-chromosome 21 genes in Table 2

From: Primary and secondary transcriptional effects in the developing human Down syndrome brain and heart

Gene name Chromosome p value Cerebrum Cerebellum Astrocyte Heart
    Microarray qPCR Microarray qPCR Microarray qPCR Microarray qPCR
Myomesin 1 (skelemin) (185 kDa) (MYOM1) 18 8.82E-08 BBL - BBL - BBL - 1.40 1.25 ± 0.06
Myoglobin (MB) 22 1.09E-07 BBL - BBL - BBL - 1.64 1.58 ± 0.16
Calsequestrin 2 (cardiac muscle) (CASQ2) 1 1.56E-07 BBL - BBL - BBL - 1.65 2.89 ± 0.58
Ras-related associated with diabetes (RRAD) 16 5.06E-06 BBL - BBL - BBL - 0.49 0.82 ± 0.08
Troponin I, cardiac (TNNI3) 19 5.90E-06 BBL - BBL - BBL - 1.62 1.31 ± 0.25
Insulin-like growth factor binding protein 7 (IGFBP7) 4 1.12E-05 1.41 1.02 ± 0.27 0.70 1.55 ± 0.33 1.74 2.12 ± 0.08 1.53 0.77 ± 0.09
Actin, alpha 1, skeletal muscle (ACTA1) 1 1.20E-05 BBL - BBL - BBL - 4.17 6.27 ± 0.59
Calcineurin-binding protein calsarcin-1 (MYOZ2) 4 1.22E-05 BBL - BBL - BBL - 1.49 1.35 ± 0.09
Cardiac troponin T2 (TNNT2) 1 2.56E-05 BBL - BBL - BBL - 1.34 1.31 ± 0.25
A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8 (ADAMTS8) 11 3.21E-05 BBL - BBL - BBL - 0.55 0.55 ± 0.17
  1. Data were normalized to the HPRT housekeeping gene. P values were derived using the same ANOVA model used for the microarray data. Values for microarray data are fold-regulation. For each qPCR experiment, values were determined by measuring samples in duplicate at multiple concentrations (mean ± standard error). Each experiment was performed independently at least three times. BBL, below background level; qPCR, quantitative real-time PCR.