Primary and secondary transcriptional effects in the developing human Down syndrome brain and heart

Table 10 Quantitative real-time PCR results for selected non-chromosome 21 genes in Table 2

Gene name	Chromosome	p value	Cerebrum		Cerebellum		Astrocyte		Heart
			Microarray	qPCR	Microarray	qPCR	Microarray	qPCR	Microarray	qPCR
Myomesin 1 (skelemin) (185 kDa) (MYOM1)	18	8.82E-08	BBL	-	BBL	-	BBL	-	1.40	1.25 ± 0.06
Myoglobin (MB)	22	1.09E-07	BBL	-	BBL	-	BBL	-	1.64	1.58 ± 0.16
Calsequestrin 2 (cardiac muscle) (CASQ2)	1	1.56E-07	BBL	-	BBL	-	BBL	-	1.65	2.89 ± 0.58
Ras-related associated with diabetes (RRAD)	16	5.06E-06	BBL	-	BBL	-	BBL	-	0.49	0.82 ± 0.08
Troponin I, cardiac (TNNI3)	19	5.90E-06	BBL	-	BBL	-	BBL	-	1.62	1.31 ± 0.25
Insulin-like growth factor binding protein 7 (IGFBP7)	4	1.12E-05	1.41	1.02 ± 0.27	0.70	1.55 ± 0.33	1.74	2.12 ± 0.08	1.53	0.77 ± 0.09
Actin, alpha 1, skeletal muscle (ACTA1)	1	1.20E-05	BBL	-	BBL	-	BBL	-	4.17	6.27 ± 0.59
Calcineurin-binding protein calsarcin-1 (MYOZ2)	4	1.22E-05	BBL	-	BBL	-	BBL	-	1.49	1.35 ± 0.09
Cardiac troponin T2 (TNNT2)	1	2.56E-05	BBL	-	BBL	-	BBL	-	1.34	1.31 ± 0.25
A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8 (ADAMTS8)	11	3.21E-05	BBL	-	BBL	-	BBL	-	0.55	0.55 ± 0.17

Data were normalized to the HPRT housekeeping gene. P values were derived using the same ANOVA model used for the microarray data. Values for microarray data are fold-regulation. For each qPCR experiment, values were determined by measuring samples in duplicate at multiple concentrations (mean ± standard error). Each experiment was performed independently at least three times. BBL, below background level; qPCR, quantitative real-time PCR.

ISSN: 1474-760X