Validation of the temporal patterns of 20E-regulated gene expression as determined by microarray analysis. (a) Northern blot hybridizations adapted with permission from published data [27,28]. Arrow indicates E74A isoform. (b) Cluster analysis of microarray data derived from RNA samples isolated from staged wild-type animals. The colors for each time point represent the change in the level of expression relative to the average expression levels across all time points for that gene, with dark blue indicating the lowest level of expression and red indicating the highest level, as depicted on the bottom. The numbers at the top indicate hours relative to pupariation, with green bars representing the peaks of 20E titer.