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Table 3 Comparison of DNA microarray data with expression data from quantitative real time PCR

From: Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert

  

Array data

Real time PCR repeat 1

Real time PCR repeat 2

GenBank ID

Gene

A

B

C

A

B

C

A

B

C

AJ780552

Cysteine protease

6.3

26.8

17.8

68.4

100.4

365.8

89.9

340.1

130.2

AJ780698

Cyclic nucleotide and calmodulin-regulated ion channel

4.7

8.9

7.7

21

13.6

18.4

15.3

35.3

21.9

AJ780435

Aldehyde dehydrogenase

4.0

4.3

4.8

5.2

4

3.1

6.8

11.2

15.5

AJ769631

ATP-dependent Clp protease ATP-binding subunit clpA

1.6

2.0

2.4

2.1

1.5

1.9

2

1.2

0.8

AJ778655

Alcohol dehydrogenase

0.5

0.7

1.1

0.6

0.6

1.1

0.8

1.1

1

AJ777239

Stable protein/bspA

0.4

0.2

0.5

0.3

0.1

0.03

0.3

0.1

0.05

AJ768555

Dehydration-responsive protein RD22

0.02

0.02

0.02

0.02

0.02

0.03

0.02

0.04

0.03

  1. Two biological repeats were independently reverse transcribed and amplified. The raw threshold cycle (Ct) values were normalized against a glucosidase alpha subunit standard to get normalized ΔCt values, which were used to calculate the fold change in expression between areas A, B, and C compared to area P.