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Table 3 Comparison of DNA microarray data with expression data from quantitative real time PCR

From: Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert

   Array data Real time PCR repeat 1 Real time PCR repeat 2
GenBank ID Gene A B C A B C A B C
AJ780552 Cysteine protease 6.3 26.8 17.8 68.4 100.4 365.8 89.9 340.1 130.2
AJ780698 Cyclic nucleotide and calmodulin-regulated ion channel 4.7 8.9 7.7 21 13.6 18.4 15.3 35.3 21.9
AJ780435 Aldehyde dehydrogenase 4.0 4.3 4.8 5.2 4 3.1 6.8 11.2 15.5
AJ769631 ATP-dependent Clp protease ATP-binding subunit clpA 1.6 2.0 2.4 2.1 1.5 1.9 2 1.2 0.8
AJ778655 Alcohol dehydrogenase 0.5 0.7 1.1 0.6 0.6 1.1 0.8 1.1 1
AJ777239 Stable protein/bspA 0.4 0.2 0.5 0.3 0.1 0.03 0.3 0.1 0.05
AJ768555 Dehydration-responsive protein RD22 0.02 0.02 0.02 0.02 0.02 0.03 0.02 0.04 0.03
  1. Two biological repeats were independently reverse transcribed and amplified. The raw threshold cycle (Ct) values were normalized against a glucosidase alpha subunit standard to get normalized ΔCt values, which were used to calculate the fold change in expression between areas A, B, and C compared to area P.