No inverse correlation between sense and antisense ratio changes. Scatter plots of log2 ratio of expression (M) (CSA-panned parasites over CD36-panned) for antisense oligonucleotides against sense oligonucleotides for var genes. Data are shown for ring, trophozoites and schizont stages from biological replicate 1. Oligonucleotides corresponding to var2csa are represented by open triangles and the other var genes from the FCR3 strain are displayed as black dots. Oligonucleotides with the highest log2 ratio of expression in CSA- compared with CD36-panned parasites often correspond to those with the highest corresponding ratios for antisense abundance (upper right datapoints). Similarly, several sense transcripts apparently highly upregulated in CD36 correspond to upregulated antisense oligos at the same loci (lower left datapoints). These data are not consistent with a direct transcriptional silencing role for antisense transcription.